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KMID : 0370220090530050281
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Yakhak Hoeji
2009 Volume.53 No. 5 p.281 ~ p.285
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Prediction of Retention Behavior of Alkyl Benzenes by Hydrophobicity Parameters in Reversed-Phase Column
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Lee Chang-Young
Lee Yong-Moon
Park Myung-Yong
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Abstract
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The retention of solutes in reversed-phase high-performance liquid chromatography depends on their hydrophobicity. Although the retention behaviors of alkyl benzenes have been reported so far, quite a few authors have mentioned the retention behavior of alkyl benzenes with plural hydrophobicity parameters. In this sense, we were interested in the retention behaviors of alkyl benzenes having benzene moiety and increasing alkyl chain. In this study, we therefore investigated the retention behavior of alkyl benzenes in reversed-phase high-performance liquid chromatography in order to obtain information concerning the effects of the aromatic moiety and the carbon chain on the retention mechanism by comparing their capacity factor (k’) in relation to the carbon chain length. The eluent acetonitrile (CH3CN) showed high selectivity on alkyl benzenes, showing the high difference of capacity factor (?log k’) between toluene and octyl benzene. Indeed, the ?log k’ of 80% CH3CN represented 1.42- and 4.25-times longer than 90% MeOH and 60% THF, respectively. The hydrophobicity parameters, van der Waals volume, bond constant, partition constant, π-energy effect and enthalpy were evaluated with the capacity factor (k’) of alkyl benzenes eluted on 80% CH3CN, 90% MeOH and 60% THF, respectively. The best eluent for predicting retention behavior of alkyl benzenes was 90% MeOH (R2 0.999). The three parameters, van der Waals volume, bond constant and partition constant were well coincident to log k’ by increasing alkyl benzenes. However, π-energy effect and enthalpy were severely disagreeable. Taken together, van der Waals volume, bond constant and partition constant were a reliable parameters to predict the retention behaviors of alkyl benzenes on reversed-phase column.
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KEYWORD
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retention behavior, alkyl benzene, hydrophobicity, reversed-phase, HPLC
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